Membrane protein structure determination by electron crystallography

Curr Opin Struct Biol. 2012 Aug;22(4):520-8. doi: 10.1016/ Epub 2012 May 8.


During the past year, electron crystallography of membrane proteins has provided structural insights into the mechanism of several different transporters and into their interactions with lipid molecules within the bilayer. From a technical perspective there have been important advances in high-throughput screening of crystallization trials and in automated imaging of membrane crystals with the electron microscope. There have also been key developments in software, and in molecular replacement and phase extension methods designed to facilitate the process of structure determination.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Bacterial Proteins / chemistry
  • Crystallization
  • Crystallography
  • Humans
  • Imaging, Three-Dimensional
  • Membrane Lipids / chemistry
  • Membrane Proteins / chemistry*
  • Microscopy, Electron, Transmission
  • Models, Molecular
  • Protein Binding
  • Protein Conformation
  • Software


  • Bacterial Proteins
  • Membrane Lipids
  • Membrane Proteins