The stability and activity of respiratory Complex II is cardiolipin-dependent

Biochim Biophys Acta. 2012 Sep;1817(9):1588-96. doi: 10.1016/j.bbabio.2012.04.015. Epub 2012 May 7.


Respiratory Complex II of the mitochondrial inner membrane serves as a link between the tricarboxylic acid cycle and the electron transport chain. Complex II dysfunction has been implicated in a wide range of heritable mitochondrial diseases, including cancer, by a mechanism that likely involves the production of reactive oxygen species (ROS). Using Complex II enzymes reconstituted into nanoscale lipid bilayers (nanodiscs) with varying lipid composition, we demonstrate for the first time that the phospholipid environment, specifically the presence of cardiolipin, is critical for the assembly and enzymatic activity of the complex, as well as in the curtailment of ROS production.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cardiolipins / physiology*
  • Electron Transport Complex II / chemistry
  • Electron Transport Complex II / metabolism*
  • Enzyme Stability
  • Mitochondria / metabolism
  • Phosphatidylglycerols / physiology
  • Reactive Oxygen Species / metabolism


  • Cardiolipins
  • Phosphatidylglycerols
  • Reactive Oxygen Species
  • respiratory complex II
  • Electron Transport Complex II