microRNA-210 negatively regulates LPS-induced production of proinflammatory cytokines by targeting NF-κB1 in murine macrophages

FEBS Lett. 2012 Apr 24;586(8):1201-7. doi: 10.1016/j.febslet.2012.03.011. Epub 2012 Mar 23.


Ligation of TLR4 with LPS in macrophages leads to the production of proinflammatory cytokines, which are central to eliminate viral and bacterial infection. However, uncontrolled TLR4 activation may contribute to pathogenesis of inflammatory diseases such as septic shock. In this study, we found microRNA-210 was induced in murine macrophages by LPS. Transfection of miR-210 mimics significantly inhibited LPS-induced production of inflammatory cytokines. In contrast, transfection of anti-miR-210 inhibitors increased LPS-induced expression of proinflammatory cytokines. Furthermore, we demonstrated that miR-210 targets NF-κB1. Therefore, our data identify miR-210 as a very important feedback negative regulator for LPS-induced production of proinflammatory cytokines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cytokines / biosynthesis*
  • Cytokines / immunology
  • HEK293 Cells
  • Humans
  • Inflammation / genetics
  • Inflammation / metabolism*
  • Lipopolysaccharides / pharmacology
  • Mice
  • Mice, Inbred C57BL
  • MicroRNAs / immunology
  • MicroRNAs / metabolism*
  • NF-kappa B p50 Subunit / antagonists & inhibitors*
  • NF-kappa B p50 Subunit / metabolism
  • Toll-Like Receptor 4 / metabolism


  • Cytokines
  • Lipopolysaccharides
  • MIRN210 microRNA, mouse
  • MicroRNAs
  • NF-kappa B p50 Subunit
  • Toll-Like Receptor 4
  • Nfkb1 protein, mouse