Transcriptome analysis reveals critical genes and key pathways for early cotton fiber elongation in Ligon lintless-1 mutant

Genomics. 2012 Jul;100(1):42-50. doi: 10.1016/j.ygeno.2012.04.007. Epub 2012 May 2.

Abstract

Fiber length is a key determinant of cotton yield and quality. Using a monogenic dominant cotton mutant Ligon lintless-1 with extremely short fibers, we employed microarray technology and quantitative real time PCR to compare transcriptomes of Li(1) and the normal wild-type TM-1, the results showed that only a few genes differentially expressed in 0 days postanthesis (DPA) ovules and 3 DPA fibers, whereas 577 transcripts differentially expressed in 6 DPA fibers. 6 DPA is probably a key phase determining fiber elongation. Gene ontology analyses showed such processes as response to stimulus, signal transduction, and lipid metabolism were readjusted by the mutant gene. Pathway studio analysis indicated that auxin signaling and sugar signaling pathways play major roles in modulation of early fiber elongation. This work provides new insight into the mechanisms of fiber development, and offers novel genes as potential objects for genetic manipulation to achieve improvement of fiber properties.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cotton Fiber*
  • Gene Expression Profiling*
  • Gene Expression Regulation, Plant
  • Genes, Plant / genetics*
  • Gossypium / genetics*
  • Mutation / genetics
  • Oligonucleotide Array Sequence Analysis
  • Ovule / genetics
  • RNA, Plant / genetics
  • Real-Time Polymerase Chain Reaction
  • Transcriptome / genetics

Substances

  • RNA, Plant