A mass spectrometric multicenter study supports classification of preeclampsia as heterogeneous disorder

Hypertens Pregnancy. 2012;31(2):278-91. doi: 10.3109/10641955.2011.640375.

Abstract

Objective: The diagnostic value of affinity-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis to distinguish preeclampsia (PE) from matched controls was tested in a multicenter setting.

Methods: Serum samples of preeclamptic (n = 60) and healthy pregnant women (n = 66) from four centers were prospectively analyzed with predefined rule sets.

Results: Overall sample classification reached sensitivity of 0.88 and specificity of 0.73. Separate calculations for early-onset PEs only (before 34 weeks of gestation) revealed sensitivity of 0.88 and specificity of 0.89.

Conclusion: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry serum-profiling with center-wise standardization offers a fast and robust method to classify PE and contributes to the theory of PE being a heterogeneous disorder that ought to be subclassified.

Publication types

  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Case-Control Studies
  • Female
  • Gestational Age
  • Humans
  • Pre-Eclampsia / blood*
  • Pre-Eclampsia / diagnosis
  • Pregnancy
  • Prospective Studies
  • Proteome
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Proteome