Overexpression of the Qc-SNARE gene OsSYP71 enhances tolerance to oxidative stress and resistance to rice blast in rice (Oryza sativa L.)

Gene. 2012 Aug 10;504(2):238-44. doi: 10.1016/j.gene.2012.05.011. Epub 2012 May 11.

Abstract

OsSYP71 is an oxidative stress and rice blast response gene that encodes a Qc-SNARE protein in rice. Qc-SNARE proteins belong to the superfamily of SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors), which function as important components of the vesicle trafficking machinery in eukaryotic cells. In this paper, 12 Qc-SNARE genes were isolated from rice, and expression patterns of 9 genes were detected in various tissues and in seedlings challenged with oxidative stresses and inoculated with rice blast. The expression of OsSYP71 was clearly up-regulated under these stresses. Overexpression of OsSYP71 in rice showed more tolerance to oxidative stress and resistance to rice blast than wild-type plants. These results indicate that Qc-SNAREs play an important role in rice response to environmental stresses, and OsSYP71 is useful in engineering crop plants with enhanced tolerance to oxidative stress and resistance to rice blast.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Genes, Plant*
  • Molecular Sequence Data
  • Oryza / genetics
  • Oryza / microbiology*
  • Oxidative Stress*
  • Phylogeny
  • Plant Proteins / chemistry
  • Plant Proteins / genetics*
  • Plants, Genetically Modified
  • SNARE Proteins / chemistry
  • SNARE Proteins / genetics*
  • Sequence Homology, Amino Acid

Substances

  • Plant Proteins
  • SNARE Proteins