Lack of detectable metabolism for solubilized 2,3,4-trimethylpentane by rat kidney proximal tubules

Toxicol Lett. 1990 Dec;54(2-3):337-44. doi: 10.1016/0378-4274(90)90201-v.

Abstract

Primary proximal tubule suspension cultures exposed to solubilized 2,3,4-trimethylpentane (2,3,4-TMP) resulted in a linear dose response, as determined by cellular lactate dehydrogenase leakage. The EC50 for 2,3,4-TMP was 16.3 mM. Metabolite analysis by gas chromatography/mass spectrometry of supernate and cell extracts from cultures exposed to 2,3,4-TMP (12.0 mM) failed to detect the presence of metabolites. Electron-microscopic examination of proximal tubules exposed to 2,3,4-TMP indicated ultrastructural changes that included increased mitochondrial swelling, increased vesiculation, decreased microvilli and pyknotic nuclei. This study indicates that kidney proximal tubules do not appear to metabolize 2,3,4-TMP.

MeSH terms

  • Animals
  • Cells, Cultured
  • Gas Chromatography-Mass Spectrometry
  • Kidney Tubules, Proximal / enzymology
  • Kidney Tubules, Proximal / metabolism*
  • L-Lactate Dehydrogenase / metabolism
  • Male
  • Mitochondria / pathology
  • Pentanes / metabolism*
  • Pentanes / toxicity
  • Rats
  • Rats, Inbred F344

Substances

  • Pentanes
  • L-Lactate Dehydrogenase
  • 2,3,4-trimethylpentane