Estimation of allowable errors for tilt parameter determination in protein electron crystallography

Ultramicroscopy. 1990 Oct;33(4):281-5. doi: 10.1016/0304-3991(90)90045-n.


A three-dimensional reconstruction of a thin protein crystal requires an accurate assignment of amplitudes and phases in the three-dimensional reciprocal space. This assignment depends upon the tilt parameters which are experimentally determined. A theoretical estimate is made of the tolerable error in the determination of tilt parameters as a function of resolution and crystal thickness.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Crystallization
  • Densitometry
  • Least-Squares Analysis
  • Proteins / chemistry*
  • Proteins / ultrastructure
  • X-Ray Diffraction*


  • Proteins