Improved viability and activity of neutrophils differentiated from HL-60 cells by co-culture with adipose tissue-derived mesenchymal stem cells

Biochem Biophys Res Commun. 2012 Jun 22;423(1):19-25. doi: 10.1016/j.bbrc.2012.05.049. Epub 2012 May 15.


Neutropenia is a principal complication of cancer treatment. We investigated the supportive effect of adipose tissue-derived mesenchymal stem cells (AD-MSCs) on the viability and function of neutrophils. Neutrophils were derived from HL-60 cells by dimethylformamide stimulation and cultured with or without AD-MSCs under serum-starved conditions to evaluate neutrophil survival, proliferation, and function. Serum starvation resulted in the apoptosis of neutrophils and decreased cell survival. The co-culture of neutrophils and AD-MSCs resulted in cell survival and inhibited neutrophil apoptosis under serum-starved conditions. The survival rate of neutrophils was prolonged up to 72 h, and the expression levels of interferon (IFN)-α, granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor, and transforming growth factor (TGF)-β in AD-MSCs were increased after co-culture with neutrophils. AD-MSCs promoted the viability of neutrophils by inhibiting apoptosis as well as enhancing respiratory burst, which could potentially be mediated by the increased expression of IFN-α, G-CSF, and TGF-β. Thus, we conclude that the use of AD-MSCs may be a promising cell-based therapy for increasing immunity by accelerating neutrophil function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / cytology
  • Cell Differentiation
  • Cell Survival / genetics
  • Coculture Techniques*
  • Gene Expression
  • Granulocyte Colony-Stimulating Factor / immunology
  • Granulocyte-Macrophage Colony-Stimulating Factor / immunology
  • HL-60 Cells
  • Humans
  • Interferon-alpha / immunology
  • Mesenchymal Stem Cell Transplantation
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / physiology*
  • Neutropenia / therapy
  • Neutrophils / cytology
  • Neutrophils / immunology*
  • Neutrophils / metabolism
  • Reactive Oxygen Species / metabolism
  • Transforming Growth Factors / immunology


  • Interferon-alpha
  • Reactive Oxygen Species
  • Granulocyte Colony-Stimulating Factor
  • Transforming Growth Factors
  • Granulocyte-Macrophage Colony-Stimulating Factor