Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 58 (3), 221-30

Determining Long-Range Chromatin Interactions for Selected Genomic Sites Using 4C-seq Technology: From Fixation to Computation

Affiliations

Determining Long-Range Chromatin Interactions for Selected Genomic Sites Using 4C-seq Technology: From Fixation to Computation

Erik Splinter et al. Methods.

Abstract

Chromosome Conformation Capture (3C) and 3C-based technologies are constantly evolving in order to probe nuclear organization with higher depth and resolution. One such method is 4C-technology that allows the investigation of the nuclear environment of a locus of choice. The use of Illumina next generation sequencing as a detection platform for the analysis of 4C data has further improved the sensitivity and resolution of this method. Here we provide a step-by-step protocol for 4C-seq, describing the procedure from the initial template preparation until the final data analysis, interchanged with background information and considerations.

Similar articles

See all similar articles

Cited by 98 PubMed Central articles

See all "Cited by" articles

Publication types

MeSH terms

LinkOut - more resources

Feedback