2b-RAD: a simple and flexible method for genome-wide genotyping

Nat Methods. 2012 May 20;9(8):808-10. doi: 10.1038/nmeth.2023.


We describe 2b-RAD, a streamlined restriction site-associated DNA (RAD) genotyping method based on sequencing the uniform fragments produced by type IIB restriction endonucleases. Well-studied accessions of Arabidopsis thaliana were genotyped to validate the method's accuracy and to demonstrate fine-tuning of marker density as needed. The simplicity of the 2b-RAD protocol makes it particularly suitable for high-throughput genotyping as required for linkage mapping and profiling genetic variation in natural populations.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Arabidopsis / genetics
  • DNA / genetics*
  • Deoxyribonucleases, Type II Site-Specific / metabolism*
  • Genome / genetics*
  • Genotype
  • Genotyping Techniques / methods*
  • Polymorphism, Single Nucleotide / genetics
  • Restriction Mapping


  • DNA
  • Deoxyribonucleases, Type II Site-Specific