Abstract
Lipid-extracted microalgal biomass residues (LMBRs) were treated using cellulase, neutrase and alcalase in a two-step process and the resulting hydrolysates were used as a source of nutrients for the cultivation of Chlorella vulgaris under non-aerated and aerated conditions for lipid production. Aeration was favorable for cell growth and lipid accumulation and a biomass of approximately 3.28 g L(-1), lipid content of 35% and lipid productivity of 116 mg L(-1) d(-1) were obtained. Thus, the tested mode of LMBRs utilization was effective for nutrient recycling in microalgal biodiesel production.
Copyright © 2012 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aerobiosis
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Amino Acids / metabolism
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Biomass*
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Biotechnology / methods*
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Carbohydrate Metabolism
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Cells, Cultured
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Cellulase / metabolism
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Chlorella vulgaris / cytology
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Chlorella vulgaris / growth & development
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Chlorella vulgaris / metabolism*
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Culture Media / chemistry
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Enzymes / metabolism*
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Fatty Acids / metabolism
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Hydrolysis
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Lipid Metabolism
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Lipids / biosynthesis*
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Lipids / isolation & purification*
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Microalgae / cytology
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Microalgae / growth & development
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Microalgae / metabolism*
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Subtilisins / metabolism
Substances
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Amino Acids
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Culture Media
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Enzymes
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Fatty Acids
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Lipids
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Cellulase
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Subtilisins