Chitosan scaffolds with BMP-6 loaded alginate microspheres for periodontal tissue engineering

J Microencapsul. 2012;29(8):770-80. doi: 10.3109/02652048.2012.686531. Epub 2012 May 21.


The aim of this study is to develop an effective growth factor releasing scaffold-microsphere system for promoting periodontal tissue engineering. Bone morphogenetic protein-6 (BMP-6)-loaded alginate microspheres in narrow size distribution were produced by optimising electrospraying conditions. The addition of these microspheres to chitosan gels produced a novel scaffold in which not only the pore sizes and interconnectivity were preserved, but also a controlled release vehicle was generated. Loading capacity was adjusted as 50 ng or 100 ng BMP-6 for each scaffold and the controlled release behaviour of BMP-6 from chitosan scaffolds was observed during seven days. Cell culture studies were carried out with rat mesenchymal stem cells derived from bone marrow in three groups; chitosan scaffolds, chitosan scaffolds containing BMP-6-loaded alginate microspheres and chitosan scaffolds with free BMP-6 in culture medium. Results showed that controlled delivery of BMP-6 from alginate microspheres has a significant effect on osteogenic differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alginates / chemistry*
  • Animals
  • Bone Morphogenetic Protein 6 / metabolism*
  • Chitosan / chemistry*
  • Culture Media
  • Glucuronic Acid / chemistry
  • Hexuronic Acids / chemistry
  • Mesenchymal Stem Cells / metabolism
  • Microscopy, Electron, Scanning
  • Microspheres*
  • Periodontium / metabolism*
  • Rats
  • Tissue Engineering*


  • Alginates
  • Bone Morphogenetic Protein 6
  • Culture Media
  • Hexuronic Acids
  • Glucuronic Acid
  • Chitosan