SPE-MS analysis of absorption, distribution, metabolism and excretion assays: a tool to increase throughput and steamline workflow

Bioanalysis. 2012 May;4(9):1111-21. doi: 10.4155/bio.12.86.


In an effort to create faster and more efficient bioanalytical methods for drug development, many investigators have evaluated a variety of SPE-MS systems. Over the past 15 years online systems have evolved from run times of >1.5 min/sample to <10 s/sample. High-throughput SPE-MS methods for in vitro absorption, distribution, metabolism and excretion screening assays have been described by several laboratories and shown to produce results comparable to conventional LC-MS/MS systems. While quantitative analysis of small molecules in biological matrixes holds many challenges, for several applications SPE-MS methods have achieved comparable results to LC-MS/MS with the benefit of 10-30-times the throughput. Based on its distinct advantages of throughput and streamlined workflow efficiencies, SPE-MS is a useful tool for the analysis of many in vitro absorption, distribution, metabolism and excretion assays and in vivo bioanalytical studies. Further development of SPE-MS methods and analysis workflows has the potential to expand the capabilities of this technology for other challenging bioanalytical applications.

Publication types

  • Review

MeSH terms

  • Absorption
  • Animals
  • Cytochrome P-450 Enzyme Inhibitors
  • Cytochrome P-450 Enzyme System / metabolism
  • Mass Spectrometry*
  • Pharmaceutical Preparations / analysis
  • Pharmaceutical Preparations / isolation & purification
  • Pharmaceutical Preparations / metabolism*
  • Pharmacokinetics
  • Solid Phase Extraction*


  • Cytochrome P-450 Enzyme Inhibitors
  • Pharmaceutical Preparations
  • Cytochrome P-450 Enzyme System