The role of the Yap5 transcription factor in remodeling gene expression in response to Fe bioavailability

PLoS One. 2012;7(5):e37434. doi: 10.1371/journal.pone.0037434. Epub 2012 May 16.


The budding yeast Saccharomyces cerevisiae has developed several mechanisms to avoid either the drastic consequences of iron deprivation or the toxic effects of iron excess. In this work, we analysed the global gene expression changes occurring in yeast cells undergoing iron overload. Several genes directly or indirectly involved in iron homeostasis showed altered expression and the relevance of these changes are discussed. Microarray analyses were also performed to identify new targets of the iron responsive factor Yap5. Besides the iron vacuolar transporter CCC1, Yap5 also controls the expression of glutaredoxin GRX4, previously known to be involved in the regulation of Aft1 nuclear localization. Consistently, we show that in the absence of Yap5 Aft1 nuclear exclusion is slightly impaired. These studies provide further evidence that cells control iron homeostasis by using multiple pathways.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Basic-Leucine Zipper Transcription Factors / genetics
  • Basic-Leucine Zipper Transcription Factors / physiology*
  • Cation Transport Proteins / biosynthesis
  • Gene Expression
  • Gene Expression Regulation, Fungal / drug effects*
  • Gene Expression Regulation, Fungal / physiology
  • Glutaredoxins / biosynthesis
  • Homeostasis
  • Iron / metabolism
  • Iron / pharmacology*
  • Iron Overload / physiopathology*
  • Nuclear Localization Signals / physiology
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / biosynthesis
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism
  • Saccharomyces cerevisiae Proteins / physiology*
  • Transcription Factors / metabolism


  • AFT1 protein, S cerevisiae
  • Basic-Leucine Zipper Transcription Factors
  • CCC1 protein, S cerevisiae
  • Cation Transport Proteins
  • Glutaredoxins
  • Grx4 protein, S cerevisiae
  • Nuclear Localization Signals
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Yap5 protein, S cerevisiae
  • Iron