The specific contributions of cohesin-SA1 to cohesion and gene expression: implications for cancer and development

Cell Cycle. 2012 Jun 15;11(12):2233-8. doi: 10.4161/cc.20318. Epub 2012 Jun 15.

Abstract

Besides its well-established role in sister chromatid cohesion, cohesin has recently emerged as major player in the organization of interphase chromatin. Such important function is related to its ability to entrap two DNA segments also in cis, thereby facilitating long-range DNA looping which is crucial for transcriptional regulation, organization of replication factories and V(D)J recombination. Vertebrate somatic cells have two different versions of cohesin, containing Smc1, Smc3, Rad21/Scc1 and either SA1 or SA2, but their functional specificity has been largely ignored. We recently generated a knockout mouse model for the gene encoding SA1, and found that this protein is essential to complete embryonic development. Cohesin-SA1 mediates cohesion at telomeres, which is required for their replication. Telomere defects in SA1- deficient cells provoke chromosome segregation errors resulting in aneuploidy despite robust centromere cohesion. This aneuploidy could explain why heterozygous animals have an earlier onset of tumorigenesis. In addition, the genome-wide distribution of cohesin changes dramatically in the absence of SA1, and the complex shows reduced accumulation at promoters and CTCF sites. As a consequence, gene expression is altered, leading to downregulation of biological processes related to a developmental disorder linked to cohesin function, the Cornelia de Lange Syndrome (CdLS). These results point out a prominent role of cohesin-SA1 in transcriptional regulation, with clear implications in the etiology of CdLS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Transport System A / genetics
  • Amino Acid Transport System A / metabolism*
  • Aneuploidy
  • Animals
  • Binding Sites
  • CCCTC-Binding Factor
  • Cell Cycle Proteins / metabolism*
  • Cell Transformation, Neoplastic
  • Chromosomal Proteins, Non-Histone / metabolism*
  • Chromosome Segregation
  • De Lange Syndrome / etiology
  • Embryonic Development
  • Gene Expression Regulation
  • HeLa Cells
  • Humans
  • Mice
  • Mice, Knockout
  • Neoplasms / metabolism
  • Neoplasms / pathology
  • Promoter Regions, Genetic
  • Repressor Proteins / metabolism
  • Telomere / metabolism

Substances

  • Amino Acid Transport System A
  • CCCTC-Binding Factor
  • CTCF protein, human
  • Cell Cycle Proteins
  • Chromosomal Proteins, Non-Histone
  • Ctcf protein, mouse
  • Repressor Proteins
  • cohesins