Bio-functionalization of ligand-free upconverting lanthanide doped nanoparticles for bio-imaging and cell targeting

Nanoscale. 2012 Jun 21;4(12):3647-50. doi: 10.1039/c2nr30982c. Epub 2012 May 23.

Abstract

We report on the functionalization of ligand-free NaGdF(4):Er(3+), Yb(3+) upconverting nanoparticles with heparin and basic fibroblast growth factor (bFGF). These upconverting nanoparticles are used to obtain high-contrast images of HeLa cells. These images reveal that the heparin-bFGF functionalized nanoparticles show specific binding to the cell membrane.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / drug effects
  • Cell Membrane / metabolism
  • Contrast Media / chemistry*
  • Contrast Media / toxicity
  • Fibroblast Growth Factor 2 / metabolism
  • HeLa Cells
  • Heparin / metabolism
  • Humans
  • Lanthanoid Series Elements / chemistry*
  • Ligands
  • Metal Nanoparticles / chemistry*
  • Metal Nanoparticles / toxicity
  • Microscopy, Fluorescence
  • Protein Binding

Substances

  • Contrast Media
  • Lanthanoid Series Elements
  • Ligands
  • Fibroblast Growth Factor 2
  • Heparin