K-252a inhibits the response of tomato cells to fungal elicitors in vivo and their microsomal protein kinase in vitro

FEBS Lett. 1990 Nov 26;275(1-2):177-80. doi: 10.1016/0014-5793(90)81466-2.

Abstract

Two characteristic responses of plant cells to fungal elicitors, induction of phenylalanine ammonia-lyase activity and of ethylene biosynthesis, were studied in suspension-cultured tomato cells. Induction of both responses was completely blocked by 500 nM K-252a, a known inhibitor of mammalian protein kinases. About 100 nM K-252a caused half-maximal inhibition. In vitro, K-252a inhibited protein kinase activity in microsomal preparations from tomato cells. Inhibition was competitive with respect to ATP and had a Ki of about 15 nM. Thus, protein kinases sensitive to K-252a occur in plants and might be important for the plant's response to fungal elicitors.

MeSH terms

  • Carbazoles / pharmacology*
  • Cell Wall
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Ethylenes / biosynthesis*
  • Fungi / pathogenicity*
  • Glucans / pharmacology
  • Glycopeptides / pharmacology
  • Glycoside Hydrolases / pharmacology
  • Indole Alkaloids
  • Phenylalanine Ammonia-Lyase / biosynthesis*
  • Plant Proteins / metabolism
  • Plants / drug effects*
  • Plants / enzymology
  • Plants / metabolism
  • Protein Kinase Inhibitors*
  • Xylan Endo-1,3-beta-Xylosidase

Substances

  • Carbazoles
  • Ethylenes
  • Glucans
  • Glycopeptides
  • Indole Alkaloids
  • Plant Proteins
  • Protein Kinase Inhibitors
  • ethylene
  • staurosporine aglycone
  • Glycoside Hydrolases
  • Xylan Endo-1,3-beta-Xylosidase
  • Phenylalanine Ammonia-Lyase