A selective Seoul-Fluor-based bioprobe, SfBP, for vaccinia H1-related phosphatase--a dual-specific protein tyrosine phosphatase

Chem Commun (Camb). 2012 Jul 4;48(52):6553-5. doi: 10.1039/c2cc31377d. Epub 2012 May 24.

Abstract

We report a Seoul-Fluor-based bioprobe, SfBP, for selective monitoring of protein tyrosine phosphatases (PTPs). A rational design based on the structures at the active site of dual-specific PTPs can enable SfBP to selectively monitor the activity of these PTPs with a 93-fold change in brightness. Moreover, screening results of SfBP against 30 classical PTPs and 35 dual-specific PTPs show that it is selective toward vaccinia H1-related (VHR) phosphatase, a dual-specific PTP (DUSP-3).

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / methods*
  • Dual Specificity Phosphatase 3 / metabolism*
  • Electron Transport
  • Fluorescent Dyes / chemistry*
  • Fluorescent Dyes / metabolism
  • Kinetics
  • Phosphorylation
  • Sensitivity and Specificity
  • Vaccinia / enzymology
  • Vaccinia / virology
  • Vaccinia virus / enzymology*

Substances

  • Fluorescent Dyes
  • Dual Specificity Phosphatase 3