Avian influenza H9N2 seroprevalence among poultry workers in Pune, India, 2010

PLoS One. 2012;7(5):e36374. doi: 10.1371/journal.pone.0036374. Epub 2012 May 18.


Avian influenza (AI) H9N2 has been reported from poultry in India. A seroepidemiological study was undertaken among poultry workers to understand the prevalence of antibodies against AI H9N2 in Pune, Maharashtra, India. A total of 338 poultry workers were sampled. Serum samples were tested for presence of antibodies against AI H9N2 virus by hemagglutination inhibition (HI) and microneutralization (MN) assays. A total of 249 baseline sera from general population from Pune were tested for antibodies against AI H9N2 and were negative by HI assay using ≥40 cut-off antibody titre. Overall 21 subjects (21/338 = 6.2%) were positive for antibodies against AI H9N2 by either HI or MN assays using ≥40 cut-off antibody titre. A total of 4.7% and 3.8% poultry workers were positive for antibodies against AI H9N2 by HI and MN assay respectively using 40 as cut-off antibody titre. This is the first report of seroprevalence of antibodies against AI H9N2 among poultry workers in India.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agricultural Workers' Diseases / epidemiology*
  • Agricultural Workers' Diseases / immunology
  • Agricultural Workers' Diseases / virology
  • Animals
  • Antibodies, Viral / blood*
  • Hemagglutination Inhibition Tests
  • Humans
  • India / epidemiology
  • Influenza A Virus, H9N2 Subtype / immunology*
  • Influenza in Birds / epidemiology*
  • Influenza in Birds / immunology
  • Influenza in Birds / transmission
  • Influenza, Human / epidemiology*
  • Influenza, Human / immunology
  • Influenza, Human / virology
  • Neutralization Tests
  • Occupational Exposure
  • Poultry
  • Seroepidemiologic Studies


  • Antibodies, Viral