A mutagenesis-based screen to rapidly identify phosphorylation sites in mitogen-activated protein kinase substrates

Anal Biochem. 2012 Aug 15;427(2):127-9. doi: 10.1016/j.ab.2012.05.015. Epub 2012 May 23.


Identification and characterization of protein phosphorylation sites often requires mass spectrometric analysis, which is not trivial or accessible to many laboratories. Here, a targeted strategy to mutagenize putative phosphorylation sites within mitogen-activated protein kinase (MAPK) substrates is described. This employs a combination of standard type II with type IIs restriction enzymes to rapidly create individual or multiple phosphorylation site mutant versions of kinase substrates with high efficiency, thereby reducing the cost for screening mutated clones.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / chemistry*
  • Arabidopsis / genetics
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Base Sequence
  • DNA Restriction Enzymes / metabolism
  • Escherichia coli
  • High-Throughput Screening Assays*
  • Mitogen-Activated Protein Kinases / genetics
  • Mitogen-Activated Protein Kinases / metabolism*
  • Molecular Sequence Data
  • Mutagenesis
  • Phosphorylation
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism


  • Arabidopsis Proteins
  • Recombinant Proteins
  • Mitogen-Activated Protein Kinases
  • DNA Restriction Enzymes