A rhoptry antigen of Plasmodium falciparum is protective in Saimiri monkeys

Parasitology. 1990 Oct;101 Pt 2:187-92. doi: 10.1017/s0031182000063228.

Abstract

A non-polymorphic antigen associated with the rhoptry organelles of Plasmodium falciparum has been purified by immunoaffinity chromatography. The antigen, RAP-1 (rhoptry associated protein-1), which is defined by monoclonal antibodies which inhibit parasite growth in vitro, is a multi-component antigen consisting of four major proteins of 80, 65, 42 and 40 kDa and two minor proteins of 77 and 70 kDa. These proteins were electro-eluted from preparative sodium dodecyl sulphate polyacrylamide gels and protected Saimiri sciureus monkeys from a lethal blood-stage infection of P. falciparum malaria. Sera from the protected animals recognized only proteins of the RAP-1 antigen when used to probe a Western blot of total parasite protein extract, confirming that RAP-1 is responsible for eliciting the protective immune response.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Protozoan / immunology*
  • Antigens, Protozoan / isolation & purification
  • Blotting, Western
  • Chromatography, Affinity
  • Electrophoresis, Polyacrylamide Gel
  • Fluorescent Antibody Technique
  • Immune Sera / immunology
  • Malaria / prevention & control*
  • Plasmodium falciparum / immunology*
  • Protozoan Proteins / immunology*
  • Protozoan Proteins / isolation & purification
  • Protozoan Vaccines*
  • Saimiri

Substances

  • Antigens, Protozoan
  • Immune Sera
  • Protozoan Proteins
  • Protozoan Vaccines
  • rhoptry associated protein, Plasmodium