Reference intervals for reproductive hormones in prepubertal children on the automated Roche cobas e 411 analyzer

Clin Biochem. 2012 Oct;45(15):1206-12. doi: 10.1016/j.clinbiochem.2012.05.019. Epub 2012 May 23.


Objectives: To establish reference intervals for luteinizing hormone (LH), follicle-stimulating hormone (FSH), estradiol (E2), progesterone (P), total and free testosterone (T) and sex-hormone binding globulin (SHBG) in prepubertal children and to assess age- and gender-related differences.

Design and methods: A total of 948 subjects, 480 girls and 468 boys, between 1 and 11 years of age, were included in this study. All assays were performed on a Roche cobas e 411 immunoassay analyzer. Reference intervals have been evaluated according to the most recent CLSI guidelines.

Results: Median values of LH, FSH and T were significantly higher in subgroups ranging from ≥ 8 to < 11 years, for both genders. In girls of that age, reference values of E2 were significantly higher than in younger ones, and in boys of the corresponding age.

Conclusion: Established reference intervals are applicable to other laboratories that use the same instrumentation.

MeSH terms

  • Age Factors
  • Blood Chemical Analysis / standards
  • Child
  • Child, Preschool
  • Estradiol / blood*
  • Female
  • Follicle Stimulating Hormone / blood*
  • Humans
  • Infant
  • Limit of Detection
  • Luteinizing Hormone / blood*
  • Male
  • Progesterone / blood*
  • Puberty
  • Reference Standards
  • Reference Values
  • Sex Factors
  • Sex Hormone-Binding Globulin / metabolism*
  • Testosterone / blood*


  • Sex Hormone-Binding Globulin
  • Testosterone
  • Progesterone
  • Estradiol
  • Luteinizing Hormone
  • Follicle Stimulating Hormone