Abstract
Male Wistar rats received fat-free diet or diets containing 5, 10 and 30% of fat (sunflower oil + lard, 1:1) for 4 weeks. The direct relationship between dietary fat level and ethoxyresorufin O-dealkylase activity of CYP1A1, methoxyresorufin O-dealkylase activity of CYP1A2, pentoxyresorufin O-dealkylase activity of CYP2B1 and testosterone 6beta-hydroxylase activity of CYP3A was found. Activities of key enzymes of phase II xenobiotic metabolism (total activity of glutathione transferase, activity of UDP-glucuronosyle transferase) and antioxidant enzymes (catalase, glutathione peroxidase, glutathione reductase, paraoxonase-1 and heme oxygenase-1) also increased with higher dietary fat level.
MeSH terms
-
Animals
-
Antioxidants / metabolism*
-
Aryl Hydrocarbon Hydroxylases / analysis
-
Aryl Hydrocarbon Hydroxylases / metabolism*
-
Aryldialkylphosphatase / blood
-
Aryldialkylphosphatase / metabolism
-
Catalase / analysis
-
Catalase / metabolism
-
Cytochrome P-450 CYP1A1 / analysis
-
Cytochrome P-450 CYP1A1 / metabolism
-
Cytochrome P-450 CYP1A2 / analysis
-
Cytochrome P-450 CYP1A2 / metabolism
-
Cytochrome P-450 CYP2B1 / analysis
-
Cytochrome P-450 CYP2B1 / metabolism
-
Cytochrome P-450 CYP3A / analysis
-
Cytochrome P-450 CYP3A / metabolism
-
Diet, Fat-Restricted
-
Dietary Fats / administration & dosage*
-
Dietary Fats / analysis
-
Dietary Fats / metabolism
-
Glucuronosyltransferase / analysis
-
Glucuronosyltransferase / metabolism
-
Glutathione Peroxidase / analysis
-
Glutathione Peroxidase / metabolism
-
Glutathione Reductase / analysis
-
Glutathione Reductase / metabolism
-
Glutathione Transferase / analysis
-
Glutathione Transferase / metabolism
-
Heme Oxygenase-1 / analysis
-
Heme Oxygenase-1 / metabolism
-
Liver / enzymology
-
Liver / metabolism
-
Male
-
Malondialdehyde / blood
-
Malondialdehyde / metabolism
-
Microsomes, Liver / enzymology*
-
Plant Oils / administration & dosage
-
Plant Oils / metabolism*
-
Rats
-
Rats, Wistar
-
Sunflower Oil
Substances
-
Antioxidants
-
Dietary Fats
-
Plant Oils
-
Sunflower Oil
-
Malondialdehyde
-
Catalase
-
Glutathione Peroxidase
-
Aryl Hydrocarbon Hydroxylases
-
Cytochrome P-450 CYP1A1
-
Cytochrome P-450 CYP1A2
-
Cytochrome P-450 CYP2B1
-
Cytochrome P-450 CYP3A
-
Heme Oxygenase-1
-
Glutathione Reductase
-
Glucuronosyltransferase
-
UDP-glucuronosyltransferase, UGT1A7
-
Glutathione Transferase
-
Aryldialkylphosphatase
-
lard