We reported 2 cases of total port-access right lower lobectomy with hilar and mediastinal lymph nodal dissection using the da Vinci surgical system through subcostal trans-diaphragmatic approach. In case 1, the operating time was 6 hours 00 minutes, and the total blood loss was little. In case 2, the operating time was 6 hours 30 minutes, and the total blood loss was little. The da Vinci surgical system provides a high-resolution stereoscopic image and allows remote, tremor-free, and scaled control of endoscopic surgical instruments with 7 degrees of freedom. Minimally invasive robot-assisted lobectomy is an effective procedure and may add benefits for both surgeon and patients.