Synthesis and characterization of PEG-based drug-responsive biohybrid hydrogels

Macromol Rapid Commun. 2012 Aug 14;33(15):1280-5. doi: 10.1002/marc.201200203. Epub 2012 May 31.


Interactive materials being responsive to a biocompatible stimulus represent a promising approach for future therapeutic applications. In this study, we present a novel biohybrid material synthesized from biocompatible components being stimulus-responsive to the pharmaceutically approved small-molecule novobiocin. The hydrogel design is based on the gyrase B (GyrB) protein, which is covalently grafted to multi-arm polyethylene glycol (PEG) using a Michael-type addition reaction. Upon addition of the GyrB-dimerizing substance coumermycin, stable hydrogels form which can be dissolved in a dose-adjustable manner by the antibiotic novobiocin. The switchable properties of this PEG-based hydrogel are favorable for future applications in tissue engineering and as externally controlled drug depot.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminocoumarins / chemistry*
  • Biocompatible Materials / chemical synthesis
  • Biocompatible Materials / chemistry*
  • DNA Gyrase / chemistry
  • DNA Gyrase / metabolism
  • Hydrogels / chemical synthesis*
  • Hydrogels / chemistry
  • Novobiocin / chemistry*
  • Polyethylene Glycols / chemistry*
  • Topoisomerase II Inhibitors


  • Aminocoumarins
  • Biocompatible Materials
  • Hydrogels
  • Topoisomerase II Inhibitors
  • Novobiocin
  • Polyethylene Glycols
  • DNA Gyrase
  • coumermycin