A simplified method for the preparation of transcriptionally active liver nuclear extracts

DNA Cell Biol. 1990 Dec;9(10):777-81. doi: 10.1089/dna.1990.9.777.


We have developed a simplified method for the preparation of liver nuclear extracts to study gene regulation and protein-DNA interactions. This protocol uses conventional laboratory equipment and standard reagents. The liver tissue is homogenized in a low-salt solution at physiological molarity with subsequent adjustment of the molarity and purification of nuclei by density sedimentation. The nuclear extracts are transcriptionally active in a validated cell-free transcription assay and contain functional DNA-binding proteins. This protocol results in the rapid preparation of highly reproducible and active liver nuclear extracts.

MeSH terms

  • Animals
  • Base Sequence
  • Cell Nucleus / metabolism
  • Cell-Free System
  • DNA / genetics
  • DNA-Binding Proteins / genetics
  • Gene Expression Regulation
  • Liver / cytology
  • Liver / metabolism*
  • Molecular Sequence Data
  • Plasmids
  • Rats
  • Templates, Genetic
  • Transcription, Genetic*


  • DNA-Binding Proteins
  • DNA