Comparison of COX2 expression between oral squamous cell carcinoma, leukoplakia and normal mucosa

J Contemp Dent Pract. 2012 Mar 1;13(2):205-9. doi: 10.5005/jp-journals-10024-1122.

Abstract

Aim: To compare cyclooxygenase 2 expression (COX2-E) between normal, oral leukoplakia lesions and different grades of oral squamous cell carcinoma (SCC).

Materials and methods: Around 90 paraffin embedded blocks consisting of 45 SCC, 15 leukoplakia and 17 controls were selected for immunohistochemistry (IHC) for detection of COX2- E. COX2-E was divided in four grades, as A (0-10%), B (11- 40%), C (41-70%) and D (< 70%) cellularity.

Results: Mean age of the patients was 55.17 ± 18.41 (M:57.92 ± 16.87, F:52.19 ± 19.74). A significant difference was found in COX2 expression between SCC total and, basal and spinous layers of leukoplakia (p > 0.05). COX2-E in spinous layer of normal tissue was significantly lower than SCC (p = 0.000). COX2-E was significantly different in SCC grade 3 and leukoplakia (p = 0.001) and normal tissue (p = 0.000). COX2-E was significantly higher in SCC grade 3 compared to leukoplakia (basal layer) (p = 0.000).

Conclusion: We showed a significant higher COX2-E in SCC lesions compared to leukoplakias and normal controls. In our study COX2-E was not significantly different in SCC grades 1, 2 and 3 (p < 0.05).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Carcinoma, Squamous Cell / enzymology*
  • Carcinoma, Squamous Cell / pathology
  • Cyclooxygenase 2 / biosynthesis*
  • Cyclooxygenase 2 / genetics
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Leukoplakia / enzymology*
  • Male
  • Middle Aged
  • Mouth Mucosa / enzymology*
  • Mouth Neoplasms / enzymology*
  • Mouth Neoplasms / pathology
  • Neoplasm Grading
  • Paraffin Embedding
  • Statistics, Nonparametric

Substances

  • Cyclooxygenase 2