[Effect of gypenosides on DMN-induced liver fibrosis in rats]

Zhongguo Zhong Yao Za Zhi. 2012 Feb;37(4):505-8.
[Article in Chinese]

Abstract

Objective: To study the effect of gypenosides on DMN-induced liver fibrosis in rats.

Method: A rat liver fibrosis model was established by injecting DMN intraperitoneally. Four weeks later, model rats were randomly devided into three groups: the model group, the gypenosides treated group (200 mg x kg(-1)) and the colchicine treated group (0.1 mg x kg(-1)), with 10 specimens for each group. After a 2-week treatment, following parameters were observed: (1) last body weight, weight ratio between liver and spleen; (2) content of liver hydroxyproline (Hyp); (3) activity of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyltransferase (gamma-GT), content of albumin (Alb) and total bilirubin( TBiL) in serum; (4) liver pathology (Sirius red staining and HE staining); (5) activity of liver superoxide dismutase (SOD), glutathione reduced (GSH), glutathione peroxidase (GSH-Px) and content of liver maleic dialdehyde (MDA).

Result: There were classic liver cirrhosis pathological changes in model groups. Compared with the normal group, liver Hyp content, activity of serum ATL, AST, gamma-GT and content of serum TBiL, MDA of model groups significantly increased; content of serum Alb and liver GSH, activity of liver SOD and GSH-Px decreased significantly in model groups. In comparison with the model group, liver cirrhosis remarkable improved in the gypenosides group, content of liver Hyp reduced significantly (P < 0.01), which was equal to the colchicine group. Compared with the model group, liver function parameters improved markedly in the gypenosides group; liver SOD and GSH-Px activities significantly increased; MDA content reduced significantly (P < 0.05).

Conclusion: Gypenosides shows an effect in treating DMN-induced liver fibrosis in rats.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Body Weight / drug effects
  • Dimethylnitrosamine / adverse effects*
  • Glutathione / metabolism
  • Glutathione Peroxidase / metabolism
  • Gynostemma
  • Hydroxyproline / metabolism
  • Liver / drug effects
  • Liver / enzymology
  • Liver / metabolism
  • Liver / pathology
  • Liver Cirrhosis / chemically induced*
  • Liver Cirrhosis / drug therapy*
  • Liver Cirrhosis / metabolism
  • Liver Cirrhosis / pathology
  • Male
  • Malondialdehyde / metabolism
  • Organ Size / drug effects
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use
  • Rats
  • Rats, Wistar

Substances

  • Plant Extracts
  • gypenoside
  • Malondialdehyde
  • Glutathione Peroxidase
  • Glutathione
  • Dimethylnitrosamine
  • Hydroxyproline