Luteimicrobium album sp. nov., a novel actinobacterium isolated from a lichen collected in Japan, and emended description of the genus Luteimicrobium

J Antibiot (Tokyo). 2012 Aug;65(8):427-31. doi: 10.1038/ja.2012.45. Epub 2012 Jun 6.

Abstract

A novel Gram-stain-positive actinobacterium, designated RI148-Li105(T), was isolated from a lichen sample from Rishiri Island, Japan, and its taxonomic position was investigated by a polyphasic approach. 16S rRNA gene sequencing study indicated that strain RI148-Li105(T) was related to the type strain of Luteimicrobium subarcticum, with a similarity of 97.8%. Cells of strain RI148-Li105(T) exhibited a rod-coccus cycle. The diagnostic cell-wall diamino acid of this organism was lysine and the peptidoglycan type was found to be A4α. The predominant menaquinones were MK-8(H(2)) and MK-9(H(2)), and the major fatty acids were iso-C(16:0), C(17:1) ω9c and C(17:0). The DNA G+C content was 73.6 mol%. The major phenotypic characteristics of strain RI148-Li105(T) basically corresponded to those of the genus Luteimicrobium excluding the fatty acid composition. These results suggest that strain RI148-Li105(T) should be affiliated with the genus Luteimicrobium. Meanwhile, DNA-DNA hybridization and some phenotypic characteristics revealed that the strain differs from L. subarcticum. Therefore, strain RI148-Li105(T) represents a novel species of the genus Luteimicrobium, for which the name Luteimicrobium album sp. nov. is proposed. The type strain of Luteimicrobium album is RI148-Li105(T) (=NBRC 106348(T)=DSM 24866(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinobacteria / chemistry
  • Actinobacteria / genetics
  • Actinobacteria / isolation & purification*
  • Actinobacteria / ultrastructure
  • Amino Acids / analysis
  • Base Composition
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Fatty Acids / analysis
  • Japan
  • Lichens / microbiology*
  • Microscopy, Electron, Scanning
  • Phylogeny
  • RNA, Ribosomal, 16S / chemistry
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Vitamin K 2 / analysis

Substances

  • Amino Acids
  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S
  • Vitamin K 2