Growth response of axenic Entamoeba histolytica to hydrogen, carbon dioxide, and oxygen

J Protozool. 1979 May;26(2):282-6. doi: 10.1111/j.1550-7408.1979.tb02781.x.


Entamoeba histolytica required CO2 for growth in axenic culture while growth was inhibited by H2. The organism was tolerant to 5% O2 in the gas phase and it was able to detoxify products of O2 reduction in the medium. The ameba did not require a negative oxidation-reduction potential for axenic growth. However, little or no free O2 was present in media exposed to 5% O2 in the gas phase. Growth was improved by adding yeast extract to the medium.

MeSH terms

  • Animals
  • Carbon Dioxide / metabolism
  • Culture Media
  • Cysteine / metabolism
  • Entamoeba histolytica / growth & development*
  • Germ-Free Life
  • Hydrogen / metabolism
  • Oxidation-Reduction
  • Oxygen Consumption


  • Culture Media
  • Carbon Dioxide
  • Hydrogen
  • Cysteine