Increased serum clearance of oligomannose species present on a human IgG1 molecule

MAbs. Jul-Aug 2012;4(4):509-20. doi: 10.4161/mabs.20450. Epub 2012 Jul 1.


The role of Fc glycans on clearance of IgG molecule has been examined by various groups in experiments where specific glycans have been enriched or the entire spectrum of glycans was studied after administration in pre-clinical or clinical pharmacokinetic (PK) studies. The overall conclusions from these studies are inconsistent, which may result from differences in antibody structure or experimental design. In the present study a well-characterized recombinant monoclonal IgG1 molecule (mAb-1) was analyzed from serum samples obtained from a human PK study. mAb-1 was recovered from serum using its ligand cross-linked to Sepharose beads. The overall purity and recovery of all isoforms were carefully evaluated using a variety of methods. Glycans were then enzymatically cleaved, labeled using 2-aminobenzamide and analyzed by normal phase high performance liquid chromatography. The assays for recovering mAb-1 from serum and subsequent glycan analysis were rigorously qualified at a lower limit of quantitation of 15 μg/mL, thus permitting analysis to day 14 of the clinical PK study. Eight glycans were monitored and classified into two groups: (1) the oligomannose type structures (M5, M6 and M7) and (2) fucosylated biantennary oligosaccharides (FBO) structures (NGA2F, NA1F, NA2F, NA1F-GlcNAc and NGA2F-GlcNAc). We observed that the oligomannose species were cleared at a much faster rate (40%) than FBOs and conclude that high mannose species should be carefully monitored and controlled as they may affect PK of the therapeutic; they should thus be considered an important quality attribute. These observations were only possible through the application of rigorous analytical methods that we believe will need to be employed when comparing innovator and biosimilar molecules.

MeSH terms

  • Antibodies, Monoclonal / blood
  • Antibodies, Monoclonal / chemistry
  • Antibodies, Monoclonal / pharmacokinetics*
  • Chromatography, High Pressure Liquid
  • Glucans / chemistry
  • Humans
  • Immunoglobulin Fc Fragments / chemistry
  • Immunoglobulin G / blood
  • Immunoglobulin G / chemistry
  • Immunoglobulin G / metabolism*
  • Mannose / chemistry*
  • Metabolic Clearance Rate
  • Molecular Structure
  • Monte Carlo Method
  • Oligosaccharides / chemistry*
  • Time Factors
  • ortho-Aminobenzoates / chemistry


  • Antibodies, Monoclonal
  • Glucans
  • Immunoglobulin Fc Fragments
  • Immunoglobulin G
  • Oligosaccharides
  • ortho-Aminobenzoates
  • Mannose
  • anthranilamide