Selected reaction monitoring-based proteomics: workflows, potential, pitfalls and future directions

Nat Methods. 2012 May 30;9(6):555-66. doi: 10.1038/nmeth.2015.


Selected reaction monitoring (SRM) is a targeted mass spectrometry technique that is emerging in the field of proteomics as a complement to untargeted shotgun methods. SRM is particularly useful when predetermined sets of proteins, such as those constituting cellular networks or sets of candidate biomarkers, need to be measured across multiple samples in a consistent, reproducible and quantitatively precise manner. Here we describe how SRM is applied in proteomics, review recent advances, present selected applications and provide a perspective on the future of this powerful technology.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Biomarkers / analysis
  • Isotope Labeling
  • Mass Spectrometry / methods*
  • Metabolic Networks and Pathways
  • Peptide Fragments / analysis
  • Peptide Fragments / isolation & purification
  • Protein Processing, Post-Translational
  • Proteomics / methods*
  • Proteomics / trends
  • Sensitivity and Specificity
  • Signal Transduction
  • Systems Biology / trends


  • Biomarkers
  • Peptide Fragments