Mechanism of the prokaryotic transmembrane disulfide reduction pathway and its in vitro reconstitution from purified components

Angew Chem Int Ed Engl. 2012 Jul 9;51(28):6900-3. doi: 10.1002/anie.201201337. Epub 2012 Jun 5.


Making your (Dsb) connection: the redox pathway bringing reducing equivalents from bacterial cytoplasm, across the inner membrane, to the three reductive Dsb pathways in the otherwise oxidizing periplasm (see scheme; TR=thioredoxin reductase, Trx=thioredoxin) is reconstituted from purified components. Transfer of reducing equivalents across the membrane is demonstrated and underlying mechanistic details are revealed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Membrane / metabolism*
  • Cytoplasm / metabolism*
  • Disulfides / chemistry*
  • Escherichia coli / metabolism*
  • Oxidation-Reduction
  • Periplasm / metabolism*
  • Proteolipids / metabolism
  • Signal Transduction
  • Thioredoxin-Disulfide Reductase / metabolism*
  • Thioredoxins / metabolism*


  • Disulfides
  • Proteolipids
  • proteoliposomes
  • Thioredoxins
  • Thioredoxin-Disulfide Reductase