Complete genome sequencing and analysis of Saprospira grandis str. Lewin, a predatory marine bacterium

Stand Genomic Sci. 2012 Mar 19;6(1):84-93. doi: 10.4056/sigs.2445005. Epub 2012 Mar 5.

Abstract

Saprospira grandis is a coastal marine bacterium that can capture and prey upon other marine bacteria using a mechanism known as 'ixotrophy'. Here, we present the complete genome sequence of Saprospira grandis str. Lewin isolated from La Jolla beach in San Diego, California. The complete genome sequence comprises a chromosome of 4.35 Mbp and a plasmid of 54.9 Kbp. Genome analysis revealed incomplete pathways for the biosynthesis of nine essential amino acids but presence of a large number of peptidases. The genome encodes multiple copies of sensor globin-coupled rsbR genes thought to be essential for stress response and the presence of such sensor globins in Bacteroidetes is unprecedented. A total of 429 spacer sequences within the three CRISPR repeat regions were identified in the genome and this number is the largest among all the Bacteroidetes sequenced to date.

Keywords: RsbR; Saprospira grandis; gliding motility; globin-coupled sensors; predatory; rhapidosomes.