Histomorphometric analysis of new bone obtained by osteogenic periosteal distraction in ovariectomized rabbits

Oral Surg Oral Med Oral Pathol Oral Radiol. 2012 Apr;113(4):472-9. doi: 10.1016/j.tripleo.2011.04.012. Epub 2011 Jul 20.


Objective: The aim of this study was to evaluate the effects of estrogen deficiency on newly formed bone obtained by osteogenic periosteal distraction histomorphometrically.

Study design: Thirty-six female rabbits were divided into 2 groups. The experimental group underwent a bilateral ovariectomy and a sham operation was applied to the control group to equalize the stress of ovariectomy surgery. Four weeks postoperatively, a gradual distraction of the mandibular corpus was performed.

Results: In the experimental group, callus formation was delayed and the new bone was less mineralized; conversely, when histomorphometric measurements were compared statistically, there were no significant differences between the ovariectomized and sham-operated subgroups in the mean extent of newly formed bone tissue.

Conclusions: Although osteoporosis caused by the lack of estrogen has negative effects on osteogenic periosteal distraction (OPD), these negative effects do not appear to present a contraindication to OPD.

MeSH terms

  • Alveolar Ridge Augmentation / methods*
  • Animals
  • Bone and Bones / anatomy & histology*
  • Contraindications
  • Estrogens / deficiency*
  • Female
  • Mandible / anatomy & histology
  • Osteogenesis, Distraction*
  • Osteoporosis / surgery*
  • Ovariectomy
  • Periosteum
  • Rabbits


  • Estrogens