High-throughput proteomic screening identifies Chlamydia trachomatis antigens that are capable of eliciting T cell and antibody responses that provide protection against vaginal challenge

Vaccine. 2012 Jun 19;30(29):4387-93. doi: 10.1016/j.vaccine.2012.01.017.

Abstract

A comprehensive proteomic screening technology was previously used to characterize T cell responses to Chlamydia trachomatis infection. In this study, we demonstrated that T cells specific for protein antigens identified through this comprehensive technology home to the site of infection after mucosal challenge with C. trachomatis. In addition, T cell responses to these proteins were elicited in multiple genetic backgrounds. Two protein antigens, CT823 and CT144, were evaluated as vaccine candidates. When administered with AbISCO-100 adjuvant, these antigens stimulated potent CD8(+) T cell responses, polyfunctional T(H)1-polarized CD4(+) T cell responses, and high titer protein-specific T(H)1-skewed antibody responses. Vaccination with either antigen with AbISCO-100 provided long-lived protection against intravaginal challenge with C. trachomatis. Adoptive transfer of immune T cells also conferred protection in the challenge model whereas passive transfer of immune serum did not, indicating the critical role for T cell responses in control of this infection. The ability of these antigens to induce potent immune responses and provide long-lived protection in response to challenge provides a basis for the rational design of a C. trachomatis subunit vaccine.

MeSH terms

  • Adjuvants, Immunologic / administration & dosage
  • Adoptive Transfer
  • Animals
  • Antibodies, Bacterial / blood
  • Antibody Formation*
  • Antigens, Bacterial / immunology*
  • Bacterial Vaccines / immunology
  • Chlamydia Infections / prevention & control*
  • Chlamydia trachomatis / immunology*
  • Cytokines / immunology
  • Female
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Proteomics
  • Recombinant Proteins / immunology
  • T-Lymphocytes / immunology*
  • Vagina / immunology*
  • Vagina / microbiology

Substances

  • Adjuvants, Immunologic
  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Vaccines
  • Cytokines
  • Recombinant Proteins