Nanosphere lithography-based platform for developing rapid and high sensitivity microarray systems

Biochem Biophys Res Commun. 2012 Jul 6;423(3):473-7. doi: 10.1016/j.bbrc.2012.05.144. Epub 2012 Jun 5.


A novel gold nanoarray (NA)-based platform was developed for microarray applications. This novel approach is based upon the principle of nanosphere lithography and can be used for one-step antibody immobilization. The developed platform was checked by functionalizing with cysteine followed by capturing biotinylated antibody and detecting it with dye-conjugated steptravidin. An immunoassay was performed with spiked samples containing human fetuin A antigen. The minimum limits of detection (LOD) of human fetuin A for NA-based and conventional microarray platforms were 50 pg/mL and 50 ng/mL, respectively. The developed approach was highly reproducible and unlike conventional microarray approaches the use of a spotting system was omitted because immobilization was controlled and directed on the predefined arrays. This approach could be an ideal alternative for developing microarrays. And, the ease of the strategy also allows the high throughput production of the microarrays.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Gold*
  • Humans
  • Metal Nanoparticles*
  • Microarray Analysis / methods*
  • Microfluidic Analytical Techniques*
  • Nanospheres*
  • Printing*
  • Protein Array Analysis / methods
  • Sensitivity and Specificity
  • alpha-2-HS-Glycoprotein / analysis


  • alpha-2-HS-Glycoprotein
  • Gold