MicroRNA-100 regulates osteogenic differentiation of human adipose-derived mesenchymal stem cells by targeting BMPR2

FEBS Lett. 2012 Jul 30;586(16):2375-81. doi: 10.1016/j.febslet.2012.05.049. Epub 2012 Jun 7.

Abstract

Elucidation of the molecular mechanisms governing human adipose-derived mesenchymal stem cells (hASCs) osteogenic differentiation is of great importance for improving the treatment of bone-related diseases. In this study, we examined the role of microRNA (miR)-100 on the osteogenesis of hASCs. Overexpression of miR-100 inhibited osteogenic differentiation of hASCs in vitro, whereas downregulation of miR-100 enhanced the process. Target prediction analysis and dual luciferase report assay confirmed that bone morphogenetic protein receptor type II (BMPR2) was a direct target of miR-100. Furthermore, knockdown of BMPR2 by RNA interference inhibited osteogenic differentiation of hASCs, similar as the effect of upregulation miR-100. Taken together, our findings imply that miR-100 plays a negative role in osteogenic differentiation and might act through targeting BMPR2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / metabolism*
  • Amino Acid Sequence
  • Bone Morphogenetic Protein Receptors, Type II / metabolism*
  • Cell Differentiation
  • Down-Regulation
  • Gene Deletion
  • Gene Expression Regulation*
  • Humans
  • Mesenchymal Stem Cells / cytology*
  • MicroRNAs / metabolism*
  • Models, Biological
  • Molecular Sequence Data
  • Osteoblasts / cytology*
  • Osteogenesis
  • RNA Interference
  • RNA, Small Interfering / metabolism
  • Sequence Homology, Amino Acid

Substances

  • MIRN100 microRNA, human
  • MicroRNAs
  • RNA, Small Interfering
  • BMPR2 protein, human
  • Bone Morphogenetic Protein Receptors, Type II