Non-thermal nanoelectroablation of UV-induced murine melanomas stimulates an immune response

Pigment Cell Melanoma Res. 2012 Sep;25(5):618-29. doi: 10.1111/j.1755-148X.2012.01027.x.

Abstract

Non-thermal nanoelectroablation therapy completely ablates UV-induced murine melanomas. C57/BL6-HGF/SF transgenic mice were exposed to UV radiation as pups and began to develop visible melanomas 5-6 months later. We have treated 27 of these melanomas in 14 mice with nanosecond pulsed electric field (nsPEF) therapy delivering 2000 electric pulses each 100 ns long and 30 kV/cm at a rate of 5-7 pulses per second. All nanoelectroablated melanoma tumors began to shrink within a day after treatment and gradually disappeared over a period of 12-29 days. Pyknosis of nuclei was evident within 1 h of nsPEF treatment, and DNA fragmentation as detected by TUNEL staining was evident by 6 h after nsPEF treatment. In a melanoma allograft system, nsPEF treatment was superior to tumor excision at accelerating secondary tumor rejection in immune-competent mice, suggesting enhanced stimulation of a protective immune response by nsPEF-treated melanomas. This is supported by the presence of CD4(+) -T cells within treated tumors as well as within untreated tumors located in mice with other melanomas that had been treated with nanoelectroablation at least 19 days earlier.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ablation Techniques / methods*
  • Animals
  • Apoptosis / radiation effects
  • CD4-Positive T-Lymphocytes / immunology
  • Electric Stimulation Therapy / methods*
  • Immunity / immunology*
  • In Situ Nick-End Labeling
  • Melanoma / immunology*
  • Melanoma / pathology
  • Melanoma / physiopathology
  • Melanoma / therapy*
  • Melanoma, Experimental / immunology
  • Melanoma, Experimental / pathology
  • Melanoma, Experimental / physiopathology
  • Melanoma, Experimental / therapy
  • Mice
  • Mice, Inbred C57BL
  • Nanomedicine / methods*
  • Reproducibility of Results
  • Skin Neoplasms / immunology
  • Skin Neoplasms / pathology
  • Skin Neoplasms / physiopathology
  • Skin Neoplasms / therapy*
  • Skin Pigmentation / radiation effects
  • Temperature
  • Ultraviolet Rays