Rapid, accurate particle tracking by calculation of radial symmetry centers

Nat Methods. 2012 Jun 10;9(7):724-6. doi: 10.1038/nmeth.2071.

Abstract

I introduce an algorithm for subpixel localization of imaged objects based on an analytic, non-iterative calculation of the best-fit radial symmetry center. This approach yields tracking accuracies that are near theoretical limits, similarly to Gaussian fitting, but with orders-of-magnitude faster execution time, lower sensitivity to nearby particles and applicability to any radially symmetric intensity distribution. I demonstrate the method with several types of data, including super-resolution microscopy images.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Algorithms
  • Computer Simulation
  • Fluorescence
  • Image Processing, Computer-Assisted / methods*
  • Models, Theoretical*
  • Particle Size
  • Sensitivity and Specificity
  • Time Factors