SIFT web server: predicting effects of amino acid substitutions on proteins

Nucleic Acids Res. 2012 Jul;40(Web Server issue):W452-7. doi: 10.1093/nar/gks539. Epub 2012 Jun 11.


The Sorting Intolerant from Tolerant (SIFT) algorithm predicts the effect of coding variants on protein function. It was first introduced in 2001, with a corresponding website that provides users with predictions on their variants. Since its release, SIFT has become one of the standard tools for characterizing missense variation. We have updated SIFT's genome-wide prediction tool since our last publication in 2009, and added new features to the insertion/deletion (indel) tool. We also show accuracy metrics on independent data sets. The original developers have hosted the SIFT web server at FHCRC, JCVI and the web server is currently located at BII. The URL is (24 May 2012, date last accessed).

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Amino Acid Substitution*
  • Genetic Variation
  • Humans
  • INDEL Mutation
  • Internet
  • Proteins / chemistry*
  • Proteins / genetics
  • Proteins / metabolism
  • Sequence Analysis, Protein
  • Software*


  • Proteins