Synthetic mammalian riboswitches based on guanine aptazyme

Chem Commun (Camb). 2012 Jul 21;48(57):7215-7. doi: 10.1039/c2cc33140c. Epub 2012 Jun 13.

Abstract

Allosteric hammerhead ribozymes (aptazymes) that are activated by guanine were used to control mammalian gene expression in cis and in trans. Coexpression of the two mechanistically distinct riboswitches resulted in an improved dynamic range of gene expression.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Aptamers, Nucleotide / genetics
  • Aptamers, Nucleotide / metabolism*
  • CHO Cells
  • Cricetinae
  • Gene Expression Regulation*
  • Green Fluorescent Proteins / genetics
  • Guanine / metabolism*
  • HEK293 Cells
  • Humans
  • RNA, Catalytic / genetics*
  • RNA, Catalytic / metabolism
  • Riboswitch*
  • Transfection

Substances

  • Aptamers, Nucleotide
  • RNA, Catalytic
  • Riboswitch
  • enhanced green fluorescent protein
  • hammerhead ribozyme
  • Green Fluorescent Proteins
  • Guanine