Diagnosis of human parechovirus infections of the central nervous system with a commercial real-time reverse transcription-polymerase chain reaction kit and direct genotyping of cerebrospinal fluid specimens

Diagn Microbiol Infect Dis. 2012 Sep;74(1):78-80. doi: 10.1016/j.diagmicrobio.2012.05.017. Epub 2012 Jun 12.

Abstract

We screened 100 cerebrospinal fluid specimens for the human parechoviruses (HPeV) genome with the commercial parechovirus r-gene™ kit, which allows results to be available in a clinically relevant time frame. HPeV infection was diagnosed in 4 infants (<4 months) and all genotyped viruses were HPeV3.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Cerebrospinal Fluid / virology*
  • Child
  • Child, Preschool
  • Encephalitis, Viral / diagnosis*
  • Encephalitis, Viral / virology
  • Female
  • Genotype
  • Humans
  • Infant
  • Infant, Newborn
  • Male
  • Middle Aged
  • Parechovirus / classification
  • Parechovirus / genetics
  • Parechovirus / isolation & purification*
  • Picornaviridae Infections / diagnosis*
  • Picornaviridae Infections / virology
  • Real-Time Polymerase Chain Reaction / methods*
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Virology / methods
  • Young Adult