D-Dimer levels at different stages of pregnancy in Australian women: a single centre study using two different immunoturbidimetric assays

Thromb Res. 2012 Sep;130(3):e171-7. doi: 10.1016/j.thromres.2012.05.022. Epub 2012 Jun 15.


Background: To date there is minimal data available on D-Dimer levels at different stages of pregnancy.

Patients and methods: We prospectively measured D-Dimer levels in 632 consecutive pregnant women from March 2007 to January 2009. The median age of the participants was 31 years (range; 18-42) with a median weight of 78 kilograms (range; 46-137). All subjects were investigated during each trimester with two different immunoturbidimetric assays; D-Dimer PLUS and INNOVANCE D-Dimer. D-Dimer levels were determined using a Sysmex® CA 1500 analyser.

Results: Our data demonstrate that D-Dimer levels in pregnancy show different patterns of rise within the first trimester, depending on the assay used; D-Dimer PLUS=0.88 (SD: mean ratio), INNOVANCE D-Dimer=0.72 (SD: mean ratio). Furthermore, the rise in mean results was greater for the INNOVANCE D-Dimer assay compared to the D-Dimer PLUS assay as shown by the ratio of third to first trimester results of 3.68 and 1.96 respectively. Both D-Dimer assays demonstrated moderate levels of intra-subject variability, with overall mean CVs of 16.5% (D-Dimer PLUS) and 16.9% (INNOVANCE D-Dimer). Furthermore, we studied the association between D-Dimer levels and occurrence of diseases of pregnancy. For both assays, there was no consistently interpretable evidence of an association between raised mean D-Dimer levels or rising D-Dimer levels and any of the diseases or conditions associated with pregnancy.

Conclusion: Our data suggest that the INNOVANCE D-Dimer assay increases significantly with the advancement of pregnancy, and is more sensitive than D-Dimer PLUS assay in the pregnant population.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Australia / epidemiology
  • Blood Chemical Analysis / statistics & numerical data
  • Female
  • Fibrin Fibrinogen Degradation Products / analysis*
  • Humans
  • Immunoassay / methods*
  • Immunoassay / statistics & numerical data*
  • Nephelometry and Turbidimetry / methods*
  • Nephelometry and Turbidimetry / statistics & numerical data*
  • Pregnancy
  • Pregnancy Complications, Cardiovascular / blood*
  • Pregnancy Complications, Cardiovascular / epidemiology*
  • Pregnancy Trimesters
  • Prevalence
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Young Adult


  • Fibrin Fibrinogen Degradation Products
  • fibrin fragment D