Production and characterization of monoclonal antibodies to the hemocytes of mud crab, Scylla serrata

Brajendu Kumar; A Deepika; M Makesh; C S Purushothaman; K V Rajendran

doi:10.1016/j.jip.2012.06.001

Production and characterization of monoclonal antibodies to the hemocytes of mud crab, Scylla serrata

J Invertebr Pathol. 2012 Sep 15;111(1):86-9. doi: 10.1016/j.jip.2012.06.001. Epub 2012 Jun 15.

Authors

Brajendu Kumar¹, A Deepika, M Makesh, C S Purushothaman, K V Rajendran

Affiliation

¹ Central Institute of Fisheries Education, Versova, Andheri (W), Mumbai 400 061, India.

PMID: 22705108
DOI: 10.1016/j.jip.2012.06.001

Abstract

Monoclonal antibodies (MAbs) to hemocytes of mud crab, Scylla serrata, were produced by immunizing mice with formalin-fixed hemocytes. Of the six MAbs produced, two (MAb 1D11 and MAb 1A2) reacted specifically with hemocyte proteins in western blot. MAb 1A2 showed strong immunofluorescent reaction with granular hemocytes and a weak reaction with semigranular cells. However, hyaline cells did not show any reaction. The MAbs also showed strong cross-reactivity with the hemocytes of different crab species but not with other crustaceans. The MAbs produced can be used as a marker for granular hemocytes of crabs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Monoclonal / immunology*
Brachyura / immunology*
Hemocytes / immunology*
Hemolymph / immunology*
Immunoglobulin Isotypes
Mice

Substances

Antibodies, Monoclonal
Immunoglobulin Isotypes