Large-scale mapping of branchpoints in human pre-mRNA transcripts in vivo

Nat Struct Mol Biol. 2012 Jun 17;19(7):719-21. doi: 10.1038/nsmb.2327.


We present the first large-scale identification of lariats-the transient branched introns that are released as a byproduct of pre-mRNA splicing. The locations of the branchpoints in these introns provide insight into the early steps of splicing. From this data set, we have developed a comprehensive model of 3' splice-site selection, identified new mechanisms of alternative splicing and mapped the distribution of splicing factors around branchpoints.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Humans
  • Introns
  • RNA Precursors / genetics*
  • RNA, Messenger / genetics*


  • RNA Precursors
  • RNA, Messenger