Quantitative analysis of miroestrol and kwakhurin for standardisation of Thai miracle herb 'Kwao Keur' (Pueraria mirifica) and establishment of simple isolation procedure for highly estrogenic miroestrol and deoxymiroestrol

Nat Prod Res. 2013 Mar;27(4-5):371-8. doi: 10.1080/14786419.2012.695370. Epub 2012 Jun 18.

Abstract

Quantitative analysis of miroestrol (1) and kwakhurin (3) by HPLC, leading to standardisation of commercially available Thai miracle herb 'Kwao Keur' which has been identified with Pueraria mirifica, was established using independent solvent systems. The simple isolation procedure of highly estrogenic miroestrol (1) and deoxymiroestrol (2) from P. mirifica was also proposed.

MeSH terms

  • Chromatography, High Pressure Liquid
  • Coumarins / chemistry
  • Coumarins / isolation & purification
  • Isoflavones / chemistry*
  • Pueraria / chemistry*
  • Steroids / chemistry*
  • Steroids / isolation & purification*

Substances

  • Coumarins
  • Isoflavones
  • Steroids
  • deoxymiroestrol
  • kwakhurin
  • miroestrol