Immobilon-P transfer membrane: applications and utility in protein biochemical analysis

Biotechniques. 1990 Dec;9(6 Suppl):788-805.

Abstract

Polyvinylidene fluoride membrane for protein-blotting applications was introduced by Millipore Corporation in 1986. The membrane, designated variously as Immobilon-PVDF, Immobilon-P Transfer Membrane and Immobilon-P, has been widely used in protein biochemical analysis. Applications developed using Millipore's polyvinylidene fluoride membrane span immunochemistry, cell biology and protein structural characterization. Additionally, protein analysis on Immobilon-P membrane has been interfaced to a range of separation methodologies including high-pressure liquid chromatography and both one- and two-dimensional electrophoresis.

Publication types

  • Review

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / analysis
  • Animals
  • Databases, Factual
  • Electrophoresis
  • Immunoassay
  • Immunoblotting
  • Membranes, Artificial*
  • Polyvinyls*
  • Protein Processing, Post-Translational
  • Proteins / analysis*
  • Proteins / chemistry
  • Proteins / metabolism

Substances

  • Amino Acids
  • Membranes, Artificial
  • Polyvinyls
  • Proteins
  • polyvinylidene fluoride