3D adaptive optics in a light sheet microscope

Opt Express. 2012 Jun 4;20(12):13252-61. doi: 10.1364/OE.20.013252.


We report on a single plane illumination microscope (SPIM) incorporating adaptive optics in the imaging arm. We show how aberrations can occur from the sample mounting tube and quantify the aberrations both experimentally and computationally. A wavefront sensorless approach was taken to imaging a green fluorescent protein (GFP) labelled transgenic zebrafish. We show improvements in image quality whilst recording a 3D "z-stack" and show how the aberrations come from varying depths in the fish.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calibration
  • Green Fluorescent Proteins / metabolism
  • Imaging, Three-Dimensional / instrumentation*
  • Light*
  • Lighting
  • Microscopy / instrumentation*
  • Optics and Photonics / instrumentation*
  • Plastics
  • Refractometry
  • Zebrafish


  • Plastics
  • Green Fluorescent Proteins